Pgex4T1 Vector. The recombinant plasmid was selected with colony pcr and size difference between intact and recombinant purificated vectors. Bacterial vector for expressing fusion proteins with a thrombin site.
Collectively, the pgex vectors provide all three translational reading frames beginning with the ecor i restriction site. I am using pgex4t1 vector for my cloning work. I inserted my gene of interest into pgex4t1 and tronsformed into bl21.
(Affinity)(Color Yellow) Copy Number :
Bacterial vector for expressing fusion proteins with a thrombin site. All pgex vectors are also engineered with an internal laciq gene. Thrombin or factor xa protease sites to cleave protein from fusion.
Collectively, The Pgex Vectors Provide All Three Translational Reading Frames Beginning With The Ecor I Restriction Site.
Collectively, the pgex vectors provide all three translational reading frames beginning with the ecori restriction site. Efficient cleavage requires at least two copies of the pluti recognition sequence. I inserted my gene of interest into pgex4t1 and tronsformed into bl21.
99/100, Based On 1 Pubmed Citations.
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. The efficiency of termination is increased by using 2 or 3 stop codons in series. The blotted membrane was blocked with 2.5% (w/v) bsa in tbst buffer (0.5m nacl,
Over 600 E3 Ligases Have Been Identified In Mammals And Most Are Part Of The Ring Family Of E3 Ligases.
This is a free resource for the scientific community that is compiled by addgene. The process of ubiquitination involves three classes of enzymes: I have cloned human genes many times earlier in this vector and had no problem.
The Gel Was Blotted On To Poly Vinylidine Difluoride (Pvdf Membrane, Roche Diagnostic) Membrane Using Transfer Buffer Containing 25 Mm Tris (Ph 8.3), 192 Mm Glycine And %20 Methanol At 90 Volts For 1.5 Hours At 4°C.
Ge healthcare pgex 4t1 vector pgex 4t1 vector, supplied by ge healthcare, used in various techniques. Ube2d2, e2 (17)kb2, pubc1, ubc4, ubc4/5, ubch4, ubch5b, ubiquitin conjugating enzyme e2 d2. The protein kinase site is located between the gst domain and the mcs.